食品科学 ›› 2022, Vol. 43 ›› Issue (6): 17-26.doi: 10.7506/spkx1002-6630-20210324-298

• 食品化学 • 上一篇    下一篇

β-酸-番茄红素/HP-β-CD复合包合物的制备及稳定性

李万茸,田秉仁,许丹,王杰,程建华,刘玉梅   

  1. (1.新疆大学化学学院,新疆乌鲁木齐 830046;2.新疆大学化工学院,煤炭清洁转化与化工过程自治区重点实验室,新疆乌鲁木齐 830046)
  • 出版日期:2022-03-25 发布日期:2022-03-28
  • 基金资助:
    国家自然科学基金地区科学基金项目(31660490)

Preparation and Stability of β-Acids-Lycopene/HP-β-CD Inclusion Complex

LI Wanrong, TIAN Bingren, XU Dan, WANG Jie, CHENG Jianhua, LIU Yumei   

  1. (1. College of Chemistry, Xinjiang University, ürümqi 830046, China; 2. Key Laboratory of Coal Cleaning Conversion and Chemical Engineering Process, Xinjiang Uyghur Autonomous Region, School of Chemical Engineering and Technology, Xinjiang University, ürümqi 830046, China)
  • Online:2022-03-25 Published:2022-03-28

摘要: 研磨法制备β-酸-番茄红素/羟丙基-β-环糊精(hydroxypropyl-β-cyclodextrin,HP-β-CD)复合包合物,采用紫外-可见光谱、傅里叶变换红外光谱、X射线粉末衍射、扫描电镜及核磁共振等方法对其结构进行表征,评价该复合包合物溶液中β-酸和番茄红素在光、热和酸碱性环境下的稳定性。结果表明,成功制备β-酸-番茄红素/HP-β-CD复合包合物,且明显改善β-酸和番茄红素的水溶性;其中,β-酸的溶解度可达3.47?mg/mL,番茄红素可达0.53?mg/mL。相较于紫外光照射和自然光条件下,复合包合物溶液避光放置16?d后,其中β-酸和番茄红素的保留率分别在80%和60%以上;而在10~90?℃的温度范围内持续保温12?h后,也均可保留70%以上。在酸性条件下,β-酸会结晶析出,因此较低的pH值(pH<5)会对复合包合物中β-酸的稳定性产生明显影响,而酸碱对番茄红素的影响不明显。上述结果表明,β-酸-番茄红素/HP-β-CD复合包合物对改善β-酸和番茄红素的光、热稳定性有积极的影响,该复合包合物更适宜用于中性和偏碱性的食品体系。

关键词: β-酸;番茄红素;羟丙基-β-环糊精;复合包合物;稳定性

Abstract: β-acids-lycopene/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex was prepared by a grinding method, and its structure was characterized by ultraviolet/visible (UV-Vis) absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and nuclear magnetic resonance (NMR). Then the stability of β-acids and lycopene in an aqueous solution of the inclusion complex was evaluated under light, thermal and acidic-alkaline conditions. The results showed that the inclusion complex was successfully prepared, which significantly increased the water solubility of β-acids and lycopene to 3.47 and 0.53 mg/mL, respectively. The inclusion complex could retain more than 80% of β-acids and 60% of lycopene after 16 days of storage in the dark compared to UV and natural light, and more than 70% of both compounds were retained in the inclusion complex held at 10–90 ℃ for 12 h. Under acidic condition (pH < 5), β-acids was crystallized, while acid-base conditions had no significant effect on lycopene. These results indicated that HP-β-CD inclusion had a positive effect on improving the photothermal stability of β-acids and lycopene, and it could be more suitable for neutral and alkaline foods.

Key words: β-acids; lycopene; HP-β-CD; inclusion complex; stability

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