食品科学 ›› 2023, Vol. 44 ›› Issue (6): 57-64.doi: 10.7506/spkx1002-6630-20220710-103

• 食品化学 • 上一篇    下一篇

不同黏度多糖阿拉伯胶和瓜尔胶对肌原纤维蛋白乳化性质的影响

缪雨雁,曹燕,立静芝,吴满刚,王庆玲,葛庆丰,于海   

  1. (1.扬州大学食品科学与工程学院,江苏 扬州 225127;2.扬州大学 江苏省淮扬菜产业化工程中心,江苏 扬州 225127)
  • 出版日期:2023-03-27 发布日期:2023-03-27
  • 基金资助:
    江苏省自然科学基金青年项目(BK20200928);扬州市绿杨金凤人才项目(YZLYJF2020PHD094); 国家自然科学基金面上项目(31371792)

Effects of Arabic Gum and Guar Gum, Polysaccharides with Different Viscosity, on the Emulsifying Properties of Myofibrillar Protein

MIAO Yuyan, CAO Yan, LI Jingzhi, WU Mangang, WANG Qingling, GE Qingfeng, YU Hai   

  1. (1. College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; 2. Industrial Engineering Center for Huaiyang Cuisine of Jiangsu Province, Yangzhou University, Yangzhou 225127, China)
  • Online:2023-03-27 Published:2023-03-27

摘要: 选取两种分子质量相近、黏度相差较大的天然高分子多糖阿拉伯胶(arabic gum,AG)和瓜尔胶(guar gum,GG)分别与肌原纤维蛋白(myofibrillar protein,MP)进行复合,研究不同多糖添加量(0.1%~0.5%)对MP-多糖复合物乳液性质的影响规律。结果表明,AG和GG均可以显著改善MP的乳化性质,其中AG对乳化活性的改善效果较好,而GG更有利于乳液的稳定性。随着多糖质量分数增加,乳化活性指数和稳定性指数均呈先升高后降低趋势,AG和GG添加量分别为0.3%和0.2%时,乳化活性指数和稳定性指数达到最大值。界面蛋白含量测定结果显示,AG和GG均会导致界面蛋白含量下降,尤其是GG的作用更明显。随着AG和GG添加量增加,乳液粒径逐渐减小,且尺寸分布更加均一,添加量超过0.3%后,GG组乳液出现少量絮凝现象。同一添加量下,GG组乳液粒径明显小于AG组。流变学分析证实,所有乳液均为假塑性流体,表现出弱凝胶性质。AG添加量较少(≤0.3%)时会降低乳液黏度,并明显提高乳液的储能模量,而GG的添加使乳液表观黏度和储能模量均显著提高,与AG相比,高黏度GG制备的乳液拥有更高的黏度和储能模量,更有利于乳液的稳定。

关键词: 肌原纤维蛋白;阿拉伯胶;瓜尔胶;乳液;界面蛋白;流变学性质

Abstract: The effects of adding different amounts (0.1%–0.5%) of arabic gum (AG) or guar gum (GG), natural polysaccharides with similar molecular mass but different viscosity, on the emulsifying properties of myofibrillar protein (MP) were investigated. The results showed that both AG and GG could significantly improve the emulsifying properties of MP, and AG was more effective in improving the emulsifying activity, while GG was more conducive to the emulsion stability. Both emulsifying activity index (EAI) and emulsion stability index (ESI) initially increased and then decreased with increasing the amount of added polysaccharides, reaching maximum values when 0.3% AG or 0.2% GG was added. Both polysaccharides, especially GG, resulted in a marked reduction of the interfacial protein content. The droplet size of emulsions gradually decreased with increasing the amount of added polysaccharides, and the droplet size distribution became more uniform. When more than 0.3% GG was added, slight flocculation occurred in the emulsions. At the same concentration, GG reduced the droplet size of the emulsions compared to AG. Rheological analysis confirmed that all emulsions were pseudoplastic fluids, exhibiting weak gel properties. AG at low levels (≤ 0.3%) caused a decrease in the viscosity of the emulsions while remarkably increasing the storage modulus. In contrast, the addition of GG significantly increased the apparent viscosity and storage modulus of the emulsions. Compared with AG, the emulsion prepared with GG, with higher viscosity, exhibited greater apparent viscosity and storage modulus, which was more favorable to the emulsion stability.

Key words: myofibrillar protein; arabic gum; guar gum; emulsion; interfacial protein; rheological properties

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