用于单糖与寡糖分离的亲水色谱材料的制备及应用进展

摘要/Abstract

摘要： 糖类化合物在食品科学、医药科学、生命科学等领域中起着至关重要的作用。糖类化合物可分为单糖、寡糖和多糖，其中单糖和寡糖大多是可溶性糖，不仅可以充当结构物质，还具有抗氧化、抗癌、抗菌、抗病毒等多种生物活性。亲水作用色谱弥补了反向液相色谱在糖类化合物分离方面的不足。文章重点介绍了适合于分离分析单糖和寡糖的氨基键合固定相、酰胺类键合固定相、糖类键合固定相、两性离子键合固定相等亲水色谱柱固定相，并概述了乙腈含量、缓冲盐浓度、流动相 pH 值和柱温等色谱分离条件对亲水分离效果的影响，以期为单糖及寡糖的分离分析提供一定的参考。

关键词: 单糖, 寡糖, 分离, 色谱固定相, 亲水作用色谱

Abstract: Carbohydrates play a vital role in food science, medicine science, life science and other fields. Carbohydrates can be divided into monosaccharides, oligosaccharides and polysaccharides, among which monosaccharides and oligosaccharides are mostly soluble, which can not only serve as structural substances, but also have various biological activities such as antioxidation, anticancer, antibacterial and antiviral. Hydrophilic interaction chromatography makes up the deficiency of reverse phase liquid chromatography in the separation of carbohydrate compounds. This paper introduced the amino bonded stationary phase, amide bonded stationary phase, carbohydrate bonded stationary phase and zwitterionic bonded stationary phase suitable for the separation and analysis of monosaccharides and oligosaccharides by hydrophilic interaction chromatography, and the effects of chromatographic separation conditions such as acetonitrile content, buffer salt concentration, pH value of mobile phase and column temperature on the separation effect were also summarized, so as to provide certain reference for the separation and analysis of monosaccharides and oligosaccharides.

Key words: Monosaccharides, Oligosaccharides, Separation, Chromatographic stationary phase, Hydrophilic interaction chromatography（HILIC）

中图分类号:

张丹丹矫芮文米春孝陈菊李想汪秋宽任丹丹何云海武龙周慧. 用于单糖与寡糖分离的亲水色谱材料的制备及应用进展[J]. 食品科学.

Xiang Li Dan-Dan REN Long WU Hui ZHOU. Process in Preparation and application of hydrophilic chromatographic materials for separation of monosaccharides and oligosaccharides[J]. FOOD SCIENCE.

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