茉莉酸甲酯类生长调节剂对‘蛇龙珠’葡萄果实品质及脂氧合酶代谢途径的影响

摘要/Abstract

摘要： 茉莉酸甲酯类物质为一种新型的植物生长调节剂，在探究外源激素对果实香气影响的研究中发现其能够显著改善果实品质并调控香气，因此茉莉酸甲酯对果实香气调控机制的阐释及其应用成为研究热点。本试验以‘蛇龙珠’葡萄果实为试材，通过测定其基本理化指标，筛选最适浓度；同时结合顶空固相微萃取-气相色谱质谱（HS-SPME-GC-MS）联用技术及酶联免疫（ELASA）分析茉莉酸甲酯类物质响应的挥发性关键代谢物及关键酶活性，探究采前茉莉酸甲酯类物质处理对‘蛇龙珠’葡萄果实品质及脂氧合酶代谢途径中绿叶挥发物的影响。结果表明，0.1 mmol L-1 茉莉酸甲酯（methyl jasmonate，MeJA）和5 mmol L-1 二氢茉莉酸甲酯（methyl dihydrojasmonate，MDJA）处理可显著提高‘蛇龙珠’葡萄果实百粒重、总糖、可溶性固形物、pH、总酚及总花色苷含量，相比于对照处理组，分别提高了9.15%、27.83%、28.24%、4.80%、77.63%、85.29%和10.24%、24.01%、24.41%、8.30%、69.21%、71.57%；与对照相比，果实可滴定酸含量分别降低了40.0%及30.24%，果实品质得到显著提高和改善。并且此浓度处理后，‘蛇龙珠’葡萄果实C6类化合物含量得到了明显的提高，更加突出了其果实香气的典型性。同时，脂氧合酶代谢途径中各个关键酶活性与代谢产物之间具有显著相关性，说明较适浓度的茉莉酸甲酯类物质处理对‘蛇龙珠’葡萄果实中绿叶挥发物的积累具有积极作用。

关键词: ‘蛇龙珠’, 茉莉酸甲酯, 二氢茉莉酸甲酯, 葡萄品质, 脂氧合酶代谢

Abstract: Methyl jasmonate (MeJA) is a new type of plant growth regulator. Several investigations into the effect of exogenous hormones, particularly methyl jasmonate, on fruit aroma revealed a significant improvement in fruit quality and the ability to regulate aroma. Therefore, the study and application of methyl jasmonate in the regulation mechanism of fruit aroma has become a research hotspot. In this study, 'Cabernet Gernischt' grape fruit was used as the test material, and the optimum concentration was screened by measuring its basic physical and chemical indices after pre-harvest treatment with methyl jasmonate (MeJA) and methyl dihydrojasmonate (MDJA) at concentrations of 0.1 mmol L-1 and 5 mmol L-1, respectively. Conversely, the volatile key metabolites and key enzyme activities in relation to methyl jasmonates application were by headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and enzyme-linked immunosorbent assay (ELASA). The effects of pre-harvest methyl jasmonates treatment on fruit quality and green leaf volatiles in the lipoxygenase metabolic pathway of 'Cabernet Gernischt' grape were also investigated. The findings revealed that the application of 0.1 mmol L-1 MeJA and 5 mmol L-1 MDJA resulted in a significant enhancement in various parameters of 'Cabernet Gernischet' grape fruit, including 100-seed weight, total sugar, soluble solids, pH, total phenol and total anthocyanin content. Compared with the control treatment group, MeJA exhibited increases of 9.15 %, 27.83 %, 28.24 %, 4.80 %, 77.63 %, 85.29 %, and 10.24 %, while MDJA caused increases of 24.01 %, 24.41 %, 8.30 %, 69.21 %, 71.57 %, respectively. Furthermore, the fruit exhibited a reduction in titratable acid content by 40.0% and 30.24% for MeJA and MDJA, respectively, resulting in a significant improvement in fruit quality. Moreover, the various concentrations of MeJA and MDJA significantly increased the content of C6 compounds in 'Cabernet Gernischet' grape fruit , which highlighted the typicality of the fruit aroma. was Also, a significant correlation was observed between the enzymatic activity of each key enzyme in the lipoxygenase metabolic pathway and the corresponding metabolites, indicating that the use of methyl jasmonate at a suitable concentration can positively influenced the accumulation of green leaf volatiles in the 'Cabernet Gernischt' grape fruit.

Key words: 'Cabernet Gernischt', methyl jasmonate (MeJA), methyl dihydrojasmonate (MDJA), grape quality, lipoxygenase metabolic

中图分类号:

TS251.52

冶楠张珍吕转转杜建明张生祥李蔚. 茉莉酸甲酯类生长调节剂对‘蛇龙珠’葡萄果实品质及脂氧合酶代谢途径的影响[J]. 食品科学.

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