联苯四氮唑沙坦类药物广谱性抗体的制备及其在降压类保健食品中多残留检测ic-ELISA法的应用

doi:10.7506/spkx1002-6630-20220731-346

食品科学 ›› 2023, Vol. 44 ›› Issue (16): 331-339.doi: 10.7506/spkx1002-6630-20220731-346

联苯四氮唑沙坦类药物广谱性抗体的制备及其在降压类保健食品中多残留检测ic-ELISA法的应用

刘凤银，苏珮韵，林浩标，刘汇，陈嘉仪，卢梓程，穆洪涛，李攻科

（1.广东第二师范学院生物与食品工程学院，广东广州 510303；2.中山大学化学学院，广东广州 510006；3.华南农业大学食品学院，广东省食品质量安全重点实验室，广东广州 510642）

出版日期:2023-08-25 发布日期:2023-09-01
基金资助:
广东省食品安全重点领域研发计划项目（2019B020211001）；国家自然科学基金面上项目（21976213）；国家自然科学基金重点项目（22134007）；广州市科技计划项目（202102080339）；广东省科技创新战略专项资金项目（pdjh2021b0369）；广东第二师范学院食品科学技术校级重点学科项目；广东省高等学校中青年教师国内访问学者计划项目

Preparation of Broad-Spectrum Polyclonal Antibody and Development of an Indirect Competitive-Enzyme Linked Immunosorbent Assay for Multi-Residue Detection of Biphenyl Tetrazolium Sartans in Antihypertensive Health Foods

LIU Fengyin, SU Peiyun, LIN Haobiao, LIU Hui, CHEN Jiayi, LU Zicheng, MU Hongtao, LI Gongke

(1. College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China;2. School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China; 3. Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China)

Online:2023-08-25 Published:2023-09-01

摘要/Abstract

摘要： 建立联苯四氮唑沙坦类药物的间接竞争酶联免疫吸附检测（indirect competitive-enzyme linked immunosorbent assay，ic-ELISA）法，并用于其在降压类保健食品中的多残留检测。将坎地沙坦与牛血清白蛋白偶联制备免疫原；免疫动物获得广谱性抗体；对坎地沙坦、氯沙坦羧酸、洛沙坦钾、奥美沙坦、奥美沙坦酯、厄贝沙坦、缬沙坦和缬沙坦甲酯的半抑制浓度分别为0.2、0.2、0.7、0.04、0.6、0.3、0.9、2.4 ng/mL。降压类保健食品经甲醇超声提取、标准稀释液稀释法消除基质效应，8 种待测物的添加回收率为80.6%～120.0%，变异系数不高于14.0%，与液相色谱-串联质谱法的测定结果一致性良好（r＞0.97），表明建立的ic-ELISA法具有良好的准确度和可靠性。

关键词: 联苯四氮唑沙坦类药物；广谱性抗体；保健食品；多残留检测；间接竞争酶联免疫吸附

Abstract: An indirect competitive-enzyme linked immunosorbent assay (ic-ELISA) was established to detect the multi-residue of biphenyl tetrazolium sartans in antihypertensive health foods. Candesartan was coupled with bovine serum albumin to obtain immunogen. New Zealand white rabbits were immunized and a broad-spectrum antibody was obtained by an antibody screening assay. The half maximal inhibitory concentrations (IC50) for candesartan, losartan carboxylic acid, losartan potassium, olmesartan, olmesartan medoxomil, irbesartan, valsartan and valsartan methyl ester were 0.2, 0.2, 0.7, 0.04, 0.6, 0.3, 0.9 and 2.4 ng/mL, respectively. The samples were extracted with methanol and the matrix effect was eliminated by diluting the extract with standard solutions. The average recoveries of the eight target compounds were in the range from 80.6% to 120.0% with coefficients of variation equal to or below 14.0%. The results of ic-ELISA were highly correlated with those of liquid chromatography-tandem mass spectrometry (LC-MS/MS) (r > 0.97), indicating high accuracy and good reliability of ic-ELISA.

Key words: biphenyl tetrazolium sartans; broad-spectrum antibody; health food; multi-residue detection; indirect competitive-enzyme linked immunosorbent assay

中图分类号:

R155.1

刘凤银，苏珮韵，林浩标，刘汇，陈嘉仪，卢梓程，穆洪涛，李攻科. 联苯四氮唑沙坦类药物广谱性抗体的制备及其在降压类保健食品中多残留检测ic-ELISA法的应用[J]. 食品科学, 2023, 44(16): 331-339.

LIU Fengyin, SU Peiyun, LIN Haobiao, LIU Hui, CHEN Jiayi, LU Zicheng, MU Hongtao, LI Gongke. Preparation of Broad-Spectrum Polyclonal Antibody and Development of an Indirect Competitive-Enzyme Linked Immunosorbent Assay for Multi-Residue Detection of Biphenyl Tetrazolium Sartans in Antihypertensive Health Foods[J]. FOOD SCIENCE, 2023, 44(16): 331-339.

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联苯四氮唑沙坦类药物广谱性抗体的制备及其在降压类保健食品中多残留检测ic-ELISA法的应用

Preparation of Broad-Spectrum Polyclonal Antibody and Development of an Indirect Competitive-Enzyme Linked Immunosorbent Assay for Multi-Residue Detection of Biphenyl Tetrazolium Sartans in Antihypertensive Health Foods

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