基于转录组学分析1-MCP与EBR联合处理对鲜黄花菜采后衰老的影响

摘要/Abstract

摘要： 鲜黄花菜花蕾采后极易衰老和出现品质劣变。本文研究了不同浓度1-甲基环丙烯（1-methylcyclopropene，1- MCP）、2，4-表油菜素内酯（2,4-epibrassionolide，EBR）及其联合处理对鲜黄花菜采后衰老的影响（贮藏温度0±1 °C）。结果表明，采后使用1 L L-1 1-MCP与1 mg L-1 EBR联合处理能够比单独处理更有效地延缓鲜黄花菜的黄化、伸长、软化、开散、失重、叶绿素降解等不良变化，从而极大地维持了黄花菜花蕾的贮藏品质。转录组学分析表明，1-MCP与EBR联合处理调控了黄花菜衰老进程相关基因的转录水平，显著抑制了乙烯的生物合成和信号转导途径相关基因的转录，而激活了油菜素内酯的生物合成和信号转导途径相关基因的转录，从而改变了黄花菜体内衰老类和生长类植物激素水平的代谢平衡。1-MCP与EBR联合处理抑制了叶绿素降解途径相关基因转录，有利于保持鲜黄花菜品质。1-MCP与EBR联合处理抑制了与衰老密切相关的E3泛素连接酶基因的转录，从而延缓细胞中蛋白质降解，推迟鲜黄花菜采后衰老的生理进程。研究结果为延缓鲜黄花菜花蕾的采后衰老和保持贮藏品质提供了理论依据和实践参考。

关键词: 鲜黄花菜, 1-甲基环丙烯, 2，4-表油菜素内酯, 联合处理, 采后衰老, 转录组

Abstract: Fresh daylily flower buds are prone to senescence and quality deterioration after harvesting, we investigated the effects of different concentrations of 1-methylcyclopropene (1-MCP), 2,4-epianolide (EBR), and their combined treatments on the postharvest senescence of fresh daylily (stored at 0±1 °C). Our results indicate that the combined treatment of 1 μL L-1 1-MCP and 1 mg L-1 EBR after harvest is more effective in delaying adverse changes, such as yellowing, elongation, softening, dispersal, weight loss, and chlorophyll degradation, compared to single treatments. This combined treatment greatly maintains the storage quality of fresh daylily. Transcriptome analysis revealed that the combination of 1-MCP and EBR regulates the transcription level of genes in daylily, significantly inhibiting ethylene biosynthesis and transcription of genes related to the signal transduction pathway, while activating the biosynthesis of brassinolide and transcription of genes related to the signal transduction pathway. Thus, it changes the balance of senescence and growth plant hormone levels in daylily. The combined treatment of 1-MCP and EBR inhibited the transcription of genes related to the chlorophyll degradation pathway, which is beneficial for maintaining the quality of fresh daylily. Additionally, the combined treatment of 1-MCP and EBR inhibited the transcription of E3 ubiquitin ligase genes closely related to senescence, thereby delaying protein degradation and postponing the physiological process of postharvest senescence in fresh daylily. Our research provides a theoretical basis and practical reference for delaying the postharvest senescence and maintaining storage quality of fresh daylily flower buds.

Key words: fresh daylily, 1-Methylcyclopropene, 2,4-epianolide, combined treatment, postharvest senescence, transcriptome

中图分类号:

TS255.3

李可昕曹建康韩晨瑞孙敏敏. 基于转录组学分析1-MCP与EBR联合处理对鲜黄花菜采后衰老的影响[J]. 食品科学, 0, (): 0-0.

Ke-Xin keLI Jian-Kang Cao. Effect of combined treatment of 1-MCP and EBR on postharvest senescence of fresh daylily (Hemerocallis citrina) based on transcriptome analysis[J]. FOOD SCIENCE, 0, (): 0-0.

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