关键词: 金针菇多糖, 苯并[a]芘, 血管炎症损伤, 造血作用, 髓系细胞生成

Abstract: Flammulina velutipes polysaccharides (FVP) exhibits excellent anti-inflammatory and antioxidant effects. To further investigate the efficacy and mechanisms of FVP in alleviating vascular injury, a benzo[a]pyrene (BaP) poisoning model was established. The expression levels of vascular inflammatory factors were assessed through immunohistochemistry analysis. Flow cytometry was used to analyze the proportions of pro-inflammatory cells in peripheral blood and spleen, as well as the composition of hematopoietic stem cell (HSC) subsets in the bone marrow. The results demonstrate that FVP treatment mitigates the expression of vascular pro-inflammatory factors such as interleukin-6, monocyte chemoattractant protein-1 and intercellular cell adhesion molecule-1 induced by BaP. Moreover, FVP treatment significantly inhibits the increased numbers of monocytes, macrophages, and neutrophils in the peripheral blood and spleen of BaP-induced mice. Further analysis of bone marrow HSC subsets reveals that BaP exposure elevates the proportion of hematopoietic progenitor cells and granulocyte-macrophage progenitor cells in mouse bone marrow, while reducing the proportion of hematopoietic stem cells and common myeloid progenitor cells. Conversely, FVP treatment reverses these effects, restoring the bone marrow hematopoietic cell subsets to levels comparable to the healthy control group. Cellular-level investigations also indicate that FVP suppresses oxidative stress in Raw264.7 cells induced by BaP. Taken together, FVP may alleviates BaP-induced vascular inflammatory injury by maintaining the homeostasis of the bone marrow hematopoietic system, regulating the output of pro-inflammatory cells into the circulatory system, and downregulating the proportion of peripheral pro-inflammatory cells.

Key words: Flammulina velutipes polysaccharide, Benzo[a]pyrene, Inflammatory vascular, hemopoiesis, myeloid cell generation