荔枝落果中A型低聚原花青素抑制类甜蛋白促炎机制

摘要/Abstract

摘要： 论文以荔枝落果中的A型低聚原花青素为研究对象，探讨其对荔枝类甜蛋白促炎的抑制机理。采用乙醇提取、大孔吸附树脂纯化以及制备液相等从荔枝落果中分离得到A型原花青素纯化物（A-type oligomeric proanthocyanidins，A-OPC），并对A-OPC结构鉴定，主要成分为原花青素A2、2个A型原花青素三聚体和1个A型四聚体，纯度达88.69%。利用荔枝类甜蛋白（Litchi thaumatin-like protein，LcTLP）诱导构建RAW264.7细胞炎症模型，A-OPC的添加可抑制LcTLP诱导RAW264.7细胞的一氧化氮、细胞炎症因子白细胞介素-6和肿瘤坏死因子-α的分泌，在120 μg/mL浓度下，抑制率分别可达58.38%、86.31%和39.80%（P＜0.01）。蛋白免疫印迹分析发现，在120 μg/mL浓度下A-OPC可降低诱导型一氧化氮合酶和环氧合酶2的表达量，并显著降低p-65、IκBα、JNK、ERK和p-38的磷酸化水平，说明A-OPC通过下调核转录因子-κB和丝裂原活化蛋白激酶通路中下游蛋白的磷酸化水平，从而抑制LcTLP诱导的炎症反应。

关键词: 荔枝落果, A型低聚原花青素, 荔枝类甜蛋白, 抗炎机制

Abstract: The paper was based on the A-type oligomeric proanthocyanidins in litchi fruitlet and investigated the inhibition mechanism of pro-inflammation induced by litchi thaumatin-like protein (LcTLP). The A-type oligomeric proanthocyanidins (A-OPC) were isolated from the fruitlet of litchi using ethanol extraction, purification by macroporous absorbing resin and preparative liquid chromatography. The structure of A-OPC was identified as proanthocyanidin A2, two A-type proanthocyanidin trimers and one A-type tetramer, with a purity of 88.69%. The inflammatory cell model was constructed by LcTLP. The addition of A-OPC inhibited the secretion of nitric oxide, interleukin-6 and tumor necrosis factor-α in RAW264.7 cells at 120 μg/ mL, the inhibition rate could reach 58.38%, 86.31% and 39.80%, respectively (P < 0.01). Western blot showed that 120 μg/mL A-OPC reduced the expression of inducible nitric oxide synthase and cyclooxygenase-2 and significantly decreased the phosphorylation levels of p-65, IκBα, JNK, ERK and p-38. Therefore, A-OPC inhibited the phosphorylation levels of downstream proteins in the nuclear transcription factor-κB and mitogen-activated protein kinase pathways to inhibit the LcTLP-induced inflammatory response.

Key words: litchi fruitlet, A-type oligomeric proanthocyanidins, litchi thaumatin-like protein, anti-inflammation mechanism

中图分类号:

TS209

罗杨合曾诗蔼李洛欣谢翀闫景坤李玉婷罗英捷赵雷. 荔枝落果中A型低聚原花青素抑制类甜蛋白促炎机制[J]. 食品科学.

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