低温放电等离子体降解葡萄干中赭曲霉毒素A的研究

摘要/Abstract

摘要： 本研究采用低温放电等离子体对葡萄干中赭曲霉毒素A（Ochratoxin A, OTA）进行降解，研究了不同时间下初始浓度、放电电压对葡萄干中OTA降解率的影响。结果表明，葡萄干中初始浓度为50 μg/mL的OTA经等离子体在放电电压75 kV时处理10 min被完全降解。利用高效液相色谱-四极杆-静电场轨道阱高分辨质谱检测并鉴定经低温放电等离子体处理的OTA降解产物，结合一级和二级质谱，推测出相对分子质量分别为m/z 426.0715（B）和m/z 158.1540（C）的2种主要降解产物结构及OTA可能的降解路径。等离子体处理前后葡萄干的理化品质没有发生显著变化，挥发性物质除酸类物质中3-甲基丁酸、乙酸、辛酸含量有明显的下降，甲酸、戊酸、2-乙基己酸含量有明显上升外，大部分的酸、醛、醇和酮类物质的相对含量未出现明显变化。研究结果表明低温放电等离子体可以有效地降解OTA，并对葡萄干品质没有显著影响，可为OTA污染的食品降解研究提供参考。

关键词: 低温放电等离子体, OTA, 降解效果, 产物解析

Abstract: In this study, low-temperature discharge plasma was used to degrade Ochratoxin A (OTA) in raisins, so as to study the effects of initial concentration and discharge voltage on the OTA degradation rate in raisins at different times. The results indicated that OTA with an initial concentration of 50 μg/mL in raisins was completely degraded by plasma for 10 minutes at a discharge voltage of 75 kV. With the adoption of high performance liquid chromatography coupled with quadruple-orbitrap high resolution mass spectrometry, OTA degradation products treated with low temperature discharge plasma were detected and identified. Combined with first-level and second-level mass spectrometry, it was inferred that the relative molecular masses were m/z 426.0715 (B) and m/z 158.1540 (C), respectively. The structures of the two main degradation products and possible degradation paths for OTA were detected and identified. There was no significant change in the physico-chemical quality of raisins before and after plasma treatment, and the relative contents of most of the acids, aldehydes, alcohols and ketones did not show significant changes in the volatile substances, except for a significant decrease in the contents of 3-methylbutyric acid, acetic acid and caprylic acid in the acids, and a significant increase in the contents of formic acid, valeric acid, and 2-ethylhexanoic acid.The research results suggests that low temperature discharge plasma can effectively degrade OTA and has no significant effect on raisin quality, which can provide a reference for food degradation studies contaminated with OTA.

Key words: Low-temperature Discharge Plasma, OTA, degradation effect, product analysis

中图分类号:

TS255.44

张绍君张文乐孙守峰段宇萌王英. 低温放电等离子体降解葡萄干中赭曲霉毒素A的研究[J]. 食品科学, 2024, 45(5): 0-0.

Wen-Le ZHANG Ying WANG. Degradation of Ochratoxin A in Raisins by Low-temperature Discharge Plasma[J]. FOOD SCIENCE, 2024, 45(5): 0-0.

参考文献

[1]张洁荣.葡萄酒加工中赭曲霉毒素A含量变化规律解析及产物安全性评价[D].杨凌: 西北农林科技大学, 2022:1-2. DOI:10.27409/d.cnki.gxbnu.2022.002006.
[2] HUMANS I W G O T E O C R T, CANCER I A F R O, ORGANIZATION.W H. Some Traditional Herbal Medicines, Some Mycotoxins, Naphthalene and Styrene [M]. World Health Organization, 2002.
[3]单艳群, 王晓英.赭曲霉毒素的电化学适体传感检测[J].化学进展, 2018, 30(06):797-808
[4] RASFF database.Ochratoxin A (21.91 μg/kg-ppb) in dried raisins from China[DB/OL]. (2019-9-27)[2023-8-22]http://news.foodmate.net/2019/09/535289.html.
[5] RASFF database.Ochratoxin A (15±3 μg/kg-ppb) in dried raisins from China[DB/OL]. (2023-5-4)[2023-8-22]http://www.chnfn.com/xinwenzhongxin/guojixinwen/oumeixinwen/2023/0504/26360.html.
[6]JIN M, BING H, LIANGXIAO Z, et al.A Structure Identification and Toxicity Assessment of the Degradation Products of Aflatoxin B1 in Peanut Oil under UV Irradiation[J].Toxins, 2016, 8(11):332-
[7] 李克.电子束辐照降解玉米赤霉烯酮和赭曲霉毒素A的研究 [D]. 无锡: 江南大学, 2019:3-6.
[8]PUKKASORN P, RATPHITAGSANTI W, HARUTHAITANASAN V.Effect of ultra‐superheated steam on aflatoxin reduction and roasted peanut properties[J].Journal of the Science of Food and Agriculture, 2018, 98(8):2935-2941
[9]YUE T, GUO C, YUAN Y, et al.Adsorptive Removal of Patulin from Apple Juice Using Ca‐Alginate‐Activated Carbon Beads[J].Journal of Food Science, 2013, 78(10):1629-1635
[10]王莉, 罗颖鹏, 罗小虎, 等.臭氧降解污染小麦中呕吐毒素的效果及降解产物推测[J].食品科学, 2016, 37(18):164-170
[11]ABRUNHOSA L, SANTOS L, VENANCIO A.Degradation of Ochratoxin A by Proteases and by a Crude Enzyme of Aspergillus niger[J].Food Biotechnology, 2006, 20(3):231-242
[12]相启森, 董闪闪, 郑凯茜, 等.大气压冷等离子体在食品农药残留和真菌毒素控制领域的应用研究进展[J].轻工学报, 2022, 37(03):1-9
[13] LIAO X, LIU D, XIANG Q, et al.Inactivation mechanisms of non-thermal plasma on microbes: A review [J]. Food Control, 2017, 75: 83-91. DOI: 10.1016/j.foodcont.2016.12.021.[J].Food Control, 2017, 75:83-91
[14]李帅, 梁珊, 谷雨.辉光放电低温等离子体改性大豆分离蛋白可食膜工艺优化[J].农业工程学报, 2018, 34(14):280-287
[15] DEVI Y, THIRUMDAS R, SARANGAPANI C, et al.Influence of cold plasma on fungal growth and aflatoxins production on groundnuts [J]. Food Control, 2017, 77: 187-191. DOI: 10.1016/j.foodcont.2017.02.019.[J].Food Control, 2017, 77:187-191
[16] WIELOGORSKA E, AHMED Y, MENEELY J, et al.A holistic study to understand the detoxification of mycotoxins in maize and impact on its molecular integrity using cold atmospheric plasma treatment [J]. Food Chemistry, 2019, 301: 125-281. DOI: 10.1016/j.foodchem.2019.125281.[J].Food Chemistry, 2019, 301:125-281
[17] DASAN B G, MUTLU M, BOYACI I H.Decontamination of Aspergillus flavus and Aspergillus parasiticus spores on hazelnuts via atmospheric pressure fluidized bed plasma reactor [J]. International Journal of Food Microbiology, 2016, 216: 50-59. DOI: 10.1016/j.ijfoodmicro.2015.09.006.[J].International Journal of Food Microbiology, 2016, 216:50-59
[18] KIM P K T, TAI P H, DHEERAWAN B, et al.Non-thermal plasma for elimination of pesticide residues in mango [J]. Innovative Food ence & Emerging Technologies, 2018, 48: 164-171. DOI: 10.1016/j.ifset.2018.06.009.[J].Innovative Food ence & Emerging Technologies, 2018, 48:164-171
[19] Ekezie F G C, Sun D W, Cheng J H.A review on recent advances in cold plasma technology for the food industry: Current applications and future trends[J]. Trends in food science & technology, 2017, 69: 46-58. DOI: 10.1016/j.tifs.2017.08.007.[J].Trends in food science & technology, 2017, 69:46-58
[20]张朋杰, 张宪臣, 李云松, 等.结合超高效液相色谱-四极杆静电场轨道阱高分辨质谱法快速测定禽畜肉中种农药残留[J].食品安全质量检测学报, 2022, 13(16):5391-5400
[21]BITTNER A, CRAMER B, HARRER H, et al.Structure elucidation and in vitro cytotoxicity of ochratoxin α amide,a new degradation product of ochratoxin A[J].Mycotoxin Research, 2015, 31(2):83-90
[22]罗小虎, 李克, 王韧, 等.臭氧、电子束辐照降解玉米赤霉烯酮和赭曲霉毒素[J].食品与机械, 2017, 33(12):98-102
[23]唐洪涛, 夏蕊, 迟蕾, 等.玉米中赭曲霉毒素的辐照降解及辐解产物的毒性评价[J].中国食物与营养, 2022, 28(04):28-32
[24]WU Q, D V, HUANG L, et al.Metabolic pathways of ochratoxin A[J].Curr Drug Metab, 2011, 12(1):1-10
[25]姚娜娜、车凤斌、张婷、李永海、张谦、张辉、周嘉佳、吴雪.不同预处理对提高大果沙棘热风干燥效果的对比分析[J].现代食品科技, 2020, 36(08):28-
[26] 曹建康江, 赵玉梅.果蔬采后生理生化实验指导 [M]. 北京：中国轻工业出版社, 2007, 136-137.
[27]陈明俊, 舒启琼, 徐建飞, 等.抗褐变马铃薯品种系鉴定与筛选[J].作物学报, 2020, 46(8):9-
[28]黎永艳, 张海霞, 邱棋伟.水分测定仪测定食品中水分的含量[J].医学动物防制, 2011, 27(9):2-
[29]袁杰, 李锬, 周利, 等.基于超高效液相色谱法和色差仪法的黄芩药材质量评价[J].世界中医药, 2019, 14(11):6-
[30]张文娟, 黄瑞彬, 徐秀娟, 等.新疆葡萄干挥发性成分差异化信息分析研究[J].食品研究与开发, 2016, 37(3):6-
[31]迟蕾, 哈益明, 王锋, 等.射线对水溶液中赭曲霉毒素降解效果的研究[J].辐射研究与辐射工艺学报, 2011, 29(01):61-64
[32] 赵青, 刘述章, 童洪辉.等离子体技术及应用 [M]. 北京: 国防工业出版社, 2009.
[33]SHI H, ILELEJI K, STROSHINE R L, et al.Reduction of Aflatoxin in Corn by High Voltage Atmospheric Cold Plasma[J].Food and Bioprocess Technology, 2017, 10(6):1042-1052
[34] 何随彬, 李好磊, 李步社等.赭曲霉毒素A的毒性作用及致毒机理研究进展[J].上海畜牧兽医通讯, 2020(04): 9-12. DOI:10.14170/j.cnki.cn31-1278/s.2020.04.004.[J]., 2020, (04):9-12
[35]GAGLIANO N, DONNE I D, TORRI C, et al.Early cytotoxic effects of ochratoxin A in rat liver: A morphological,biochemical and molecular study[J].Toxicology, 2006, 225(2-3):214-224
[36]ZURICH M G, LENGACHER S, BRAISSANT O, et al.Unusual astrocyte reactivity caused by the food mycotoxin ochratoxin A in aggregating rat brain cell cultures[J].Neuroscience, 2005, 134(3):771-782
[37] ANA-MARIJA, DOMIJAN, GORAN, et al.In vitro genotoxicity of mycotoxins ochratoxin A and fumonisin B1 could be prevented by sodium copper chlorophyllin--implication to their genotoxic mechanism [J].Food chemistry, 2015, 170:455-462.DOI: 10.1016/j.foodchem.2014.08.036.[J].Food Chemistry, 2015, 170:455-462
[38]王玉萍, 黄昆仑, 梁志宏.微生物对赭曲霉毒素的生物脱毒机理研究进展[J].农业生物技术学报, 2017, 25(02):316-323
[39] 彭春红.电子束辐照对赭曲霉毒素A的降解效果及机理研究 [D]. 福州: 福建农林大学, 2015:42-48.
[40]ABRUNHOSA L, PATERSON R R, VENaNCIO A.Biodegradation of ochratoxin a for food and feed decontamination[J].Toxins, 2010, 2(5):1078-1099
[41]HAQ M, GONZALEZ N, MINTZ K, et al.Teratogenicity of Ochratoxin A and the Degradation Product,Ochratoxin α,in the Zebrafish (Danio rerio) Embryo Model of Vertebrate Development[J].Toxins, 2016, 8(2):40-
[42]HUFF W E, HAMILTON P B.Mycotoxins – Their Biosynthesis in Fungi: Ochratoxins – Metabolites of Combined Pathways[J].Journal of Food Protection, 1979, 42(10):815-820
[43] 解梦梦, 赵武奇, 贾梦科, 等.低温等离子体处理对鲜切猕猴桃片质构及理化特性的影响[J]., 2021, 21(10):133-142
[44] 邹东.诱变黑曲霉对赭曲霉毒素A的脱除作用及其在麦麸中的应用 [D]. 无锡:江南大学, 2022:27-31. DOI:10.27169/d.cnki.gwqgu.2022.000491.