关键词: 罗伊氏乳杆菌，肠道健康，miRNA，炎症，凋亡

Abstract: Objective: To investigate the effect and mechanism of Lactobacillus reuteri on inflammatory response and apoptosis of intestinal epithelial cells. Methods: Immunofluorescence and ELISA were used to detect the effect of Lactobacillus reuteri on inflammatory reaction and apoptosis of intestinal epithelial cells. Bioinformatics analysis, qPCR and Double luciferase reporter gene were used to screen the downstream miRNA of Lactobacillus reuteri. The downstream target gene of miR-196a and its function were screened by double luciferase reporter gene and Western blot. Results: Lactobacillus reuteri inhibited the inflammatory reaction and apoptosis of intestinal epithelial cells induced by lipopolysaccharide (LPS). Further analysis showed that Lactobacillus reuteri inhibited cell inflammation and apoptosis by up-regulating intestinal epithelial cell miR-196a. MiR-196a protects intestinal epithelial cells by targeting the expression of programmed cell death 4 (PDCD4). Conclusion: Lactobacillus reuteri inhibits the expression of PDCD4 gene through miR-196a to alleviates the inflammatory reaction and apoptosis of intestinal epithelial cells.

Key words: Lactobacillus reuteri, intestinal health, miRNA, inflammation, apoptosis