基于转录组学分析冰温贮藏延缓勃氏甜龙竹笋采后木质化及相关机制

摘要/Abstract

摘要： 摘要：为研究冰温（near-freezing temperature，NFT）贮藏对竹笋采后木质化的调控作用及相关机制，本研究以勃氏甜龙竹笋为研究对象，通过分析贮藏过程中硬度、呼吸强度、失重率、木质素含量变化，总结NFT在甜龙竹笋上的保鲜效果；同时根据PAL、C4H、CAD、POD酶活变化和木质素合成基因表达情况，分析NFT延缓甜龙竹笋木质化的相关机制。结果表明：NFT抑制了甜龙竹笋硬度、呼吸强度、失重率上升，稳定木质素含量，延缓了甜龙竹笋采后衰老；NFT有效稳定了PAL、CAD、C4H、POD活性；基于转录组技术筛选到苯丙烷代谢途径中58个差异表达基因，其中33个基因受NFT影响表达量下调，发现NFT下调了PAL、4CL、HCT、CCR、CAD等表达。推测NFT通过稳定PAL、CAD、C4H、POD酶活或下调PAL、4CL、HCT等木质素合成相关基因表达，稳定甜龙竹笋木质素含量，进而延缓了甜龙竹笋采后衰老。

关键词: 关键词：勃氏甜龙竹笋, 冰温贮藏, 木质化, 转录组, 采后

Abstract: Abstract: In order to investigate the effect and relevant mechanism of NFT (near-freezing temperature) on delaying bamboo shoots lignification, with D.brandisii (Dendrocalamus brandisii) shoots as material, the preservation effect of NFT on D.brandisii shoots was summarized based on the changes of hardness, respiration intensity, weight loss and lignin contents during storage; in addition, the relevant mechanism was hypothesized based on the change of PAL, C4H, CAD and POD activities and the expression of genes which are key in lignin synthesization. It was indicated that NFT effectively delayed the postharvest senescence of D.brandisii shoots, which inhibited the increase of hardness, respiratory strength and weight loss of D.brandisii shoots, maintained the lignin content of D.brandisii shoots. Furthermore, it was found that NFT stabilized PAL, CAD, C4H, and POD activities. Moreover, 58 differentially expressed genes in the phenylpropane metabolic pathway were screened based on transcriptome technology, of which 33 genes were down-regulated by NFT; among them, the transcription of PAL, 4CL, HCT, CCR, CAD, was roughly reduced by NFT. It was hypothesized that NFT might delay the postharvest senescence and maintain lignin content of D.brandisii shoots by stabilizing the activities of PAL, CAD, C4H and POD and down-regulating gene expression in the phenylpropane metabolic pathway including PAL, 4CL and HCT genes, etc.

Key words: Keywords: Dendrocalamus brandisii shoots, near-freezing temperature, lignification, transcriptome, postharvest

中图分类号:

TS255.3

杨双鹤申挥罗海波苏火生石萍萍邹琴俞源河陈亚男董霞于丽娟. 基于转录组学分析冰温贮藏延缓勃氏甜龙竹笋采后木质化及相关机制[J]. 食品科学.

Shuang-He YANG Hai-Bo LUO. Effect and mechanism of near-freezing temperature on delaying postharvest Dendrocalamus brandisii shoots lignification based on transcriptome analysis[J]. FOOD SCIENCE.

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