食品科学 ›› 2024, Vol. 45 ›› Issue (13): 104-114.doi: 10.7506/spkx1002-6630-20231027-235

• 生物工程 • 上一篇    

不同香型大曲微生态结构及其发酵特性相关性分析

徐锦, 冯文聪, 朱澳迪, 李良, 董孝元, 常煦, 陈茂彬, 方尚玲   

  1. (1.工业发酵省部共建协同创新中心,发酵工程教育部重点实验室,工业微生物湖北省重点实验室,湖北工业大学生物工程与食品学院,湖北 武汉 430068;2.黄鹤楼酒业有限公司,湖北 武汉 430050;3.湖北安琪酵母股份有限公司,湖北 宜昌 443000)
  • 发布日期:2024-07-12
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFD0400500);湖北省科技厅重大专项(2018ABA084)

Correlation Analysis between Microecological Structure and Fermentation Characteristics of Different Aroma Types of Daqu

XU Jin, FENG Wencong, ZHU Aodi, LI Liang, DONG Xiaoyuan, CHANG Xu, CHEN Maobin, FANG Shangling   

  1. (1. Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, School of Food and Biological Engineering, Hubei University of Technology, Wuhan 430068, China; 2. Yellow Crane Tower Distillery Co. Ltd., Wuhan 430050, China; 3. Hubei Angel Yeast Co. Ltd., Yichang 443000, China)
  • Published:2024-07-12

摘要: 以清香型、浓香型和酱香型白酒大曲为研究对象,采用理化分析、顶空固相微萃取气相色谱-质谱检测和高通量测序技术,探究不同香型大曲微生态与理化指标和酒质特征风味化合物的潜在关系。结果表明,清香大曲的糖化力和发酵力最高,浓香型大曲的酯化力和淀粉含量最高,酱香型大曲的酸度最高。3 种香型大曲优势细菌属包括乳酸杆菌属(Saccharopolyspora)、魏斯氏菌属(Weissella)、芽孢杆菌属(Bacillus);优势真菌属包括复膜孢酵母属(Saccharomycopsis)、曲霉属(Aspergillus)、嗜热霉菌属(Thermomyces)。相关性分析表明,芽孢杆菌属与糖化力、液化力、发酵力、癸酸乙酯、苯乙醇呈正相关;嗜热霉菌属与己酸乙酯、异戊醇、乳酸乙酯、乙酸、川芎嗪呈正相关。

关键词: 不同香型大曲;理化指标;特征风味化合物;微生物群落;相关性分析

Abstract: Physicochemical analysis, headspace-solid phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) and high-throughput sequencing were used to investigate the potential relationship between the microecology of different flavor types of Daqu and their physicochemical indexes and characteristic flavor compounds. The results showed that the highest saccharification power and fermentation power were found in Fen-flavor Daqu, the highest esterification power and starch content in Luzhou-flavor Daqu, and the highest acidity were found in Maotai-flavor Daqu. In the three types of Daqu, the dominant bacterial genera were Saccharopolyspora, Weissella and Bacillus, and the dominant fungal genera were Saccharomycopsis, Aspergillus and Thermomyces. Correlation analysis showed that Bacillus was positively correlated with saccharification power, liquefaction power, fermentation power, ethyl caprate and phenyl ethanol. Thermomyces was positively correlated with ethyl caproate, isoamyl alcohol, ethyl lactate, acetic acid and ligustrazine.

Key words: different flavor types of Daqu; physicochemical indexes; characteristic flavor compounds; microbial community; correlation analysis

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