九蒸九制黄精多糖的结构表征及降血糖活性分析

摘要/Abstract

摘要： 本文以九蒸九制的黄精为研究对象，对黄精多糖成分进行分离纯化、结构表征和降血糖活性研究。通过水提醇沉法提取得到黄精粗多糖，经DEAE-52纤维层析柱分离纯化后得到3种均一多糖分别为SPGK、SPGK2和SPGK3。通过测定3种多糖的分子量、单糖组成、傅里叶红外、甲基化气质和核磁共振对其进行结构表征，并采用酶抑制实验对3种多糖降血糖活性进行研究。结果表明，SPGK、SPGK2和SPGK3的分子量分别为12.74 kDa、68.72 kDa和10.25 kDa；SPGK主要由半乳糖组成，其结构主要包括→4)-α-D-Galp-(1→、→4)-β-D-Galp-(1→、→4,6)-β-D-Galp-(1→和T-β-D-Galp-(1→；SPGK2主要由鼠李糖、阿拉伯糖、半乳糖和葡萄糖组成，其结构主要包括→2,4)-α-L-Rhap-(1→、→5)-α-L-Araf-(1→、→4)-α-D-Galp-(1→、→4)-β-D-Galp-(1→、→4,6)-β-D-Galp-(1→、T-β-D-Glcp(1→和T-β-D-Galp-(1→；SPGK3主要由鼠李糖、半乳糖和半乳糖醛酸组成，其结构主要包括→3)-α-L-Rhap-(1→、→2,4)-α-L-Rhap-(1→、→4)-α-D-GalAp-(1→、→4)-β-D-Galp-(1→、→4,6)-β-D-Galp-(1→、→4)-β-D-Glcp-(1→和T-β-D-Galp-(1→。酶抑制实验表明SPGK、SPGK2和SPGK3均具有一定的α-淀粉酶和α-葡萄糖苷酶抑制活性，在2 mg/mL浓度下对α-淀粉酶抑制率分别为41.53%、83.60%和56.15%；对α-葡萄糖苷酶的抑制率分别为28.03%、83.97%和65.76%。综上所述，从九蒸九制黄精中提取的SPGK、SPGK2和SPGK3具有一定的降血糖潜力，为其产品开发和降血糖应用提供理论依据。

关键词: 黄精多糖, 九蒸九制, 结构表征, 降血糖活性, 构效关系

Abstract: This study focuses on the nine steaming-nine processed Polygonatum sibiricum and investigates the polysaccharide components. The research includes the separation and purification of the polysaccharides, structural characterization, and analysis of their hypoglycemic activity. The crude polysaccharides from Polygonatum sibiricum were obtained by water extraction and alcohol precipitation method, followed by purification using DEAE-52 column chromatography, resulting in three homogeneous polysaccharides named SPGK, SPGK2, and SPGK3. The structural characterization of the polysaccharides was conducted through analysis of molecular weight, monosaccharide composition, Fourier-transform infrared spectroscopy, methylation analysis, and nuclear magnetic resonance spectroscopy. Enzyme inhibition assays and HepG2 cell experiments were employed to study the hypoglycemic activity of the three polysaccharides. Results indicated that the molecular weights of SPGK, SPGK2, and SPGK3 were 12.74 kDa, 68.72 kDa, and 10.25 kDa, respectively. SPGK was primarily composed of galactose, while SPGK2 contained arabinose, galactose, rhamnose, and glucose, and SPGK3 consisted mainly of rhamnose, galactose, and galacturonic acid. Enzyme inhibition assays revealed that SPGK, SPGK2, and SPGK3 exhibited significant α-amylase and α-glucosidase inhibition activities. At a concentration of 2 mg/mL, the inhibition rates of α-amylase were 41.53%, 83.60%, and 56.15%, respectively, while the inhibition rates of α-glucosidase were 28.03%, 83.97%, and 65.76%, respectively. In a word, polysaccharides SPGK, SPGK2, and SPGK3 extracted from Polygonatum sibiricum prepared by the " Nine Steaming-Nine Processing " method exhibited promising hypoglycemic potential. This study provides valuable insights into the structural characterization of polysaccharides from Polygonatum sibiricum and offers a theoretical basis for the development and application of its products.

Key words: Polygonatum sibiricum polysaccharides, nine steaming-nine processing, structural characterization, hypoglycemic activity, conformational relationship

中图分类号:

TS201.2

宋鸿杰闫新旭冯宝龙王喜波王玉堂. 九蒸九制黄精多糖的结构表征及降血糖活性分析[J]. 食品科学.

baolong feng Xi-bo Wang 玉堂王. Structural Characterization and Hypoglycemic Activity of Polysaccharides from Polygonatum sibiricum by Nine Steaming-Nine Processing[J]. FOOD SCIENCE.

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