Determination of Gibberellin Residue in Vegetables and Fruits by HPLC-MS/MS

doi:10.7506/spkx1002-6630-201106048

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (6 ): 209-212.doi: 10.7506/spkx1002-6630-201106048

• Analysis & Detection • Previous Articles Next Articles

Determination of Gibberellin Residue in Vegetables and Fruits by HPLC-MS/MS

ZHAO Ying-bo1，ZHOU Yan-ming1,*，XIN Xue1,2，WANG Yan-song3

1. Analysis Test Center, Shenyang Agricultural University, Shenyang 110866, China；
2. Institute of Shenyang Grain and Oil Metering, Shenyang 110021, China；
3. Shenyang Product Quanlity Supervision and Inspection Institute, Shenyang 110022, China

Received:2010-06-03 Revised:2011-01-25 Online:2011-03-25 Published:2011-03-03
Contact: ZHAO Ying-bo1 E-mail:yb_zhao1979@163.com

Abstract

Abstract: A rapid and sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of gibberellin residue in vegetables and fruits was developed. The gibberellin in samples was primarily extracted by methanol solution and further extracted by ethyl acetate after pH adjustment from 3 to 4. The extract was purified by C18 column and detected by HPLC-MS/MS with quantification through an external standard method. Linear range of gibberellin in this determination method was between 0.01μg/mL and 10.0μg/mL, and the detection limit was 0.01 μg/mL. The recovery rates of gibberellin in all samples at a spiked level ranging from 0.02 to 1.0 mg/kg were in the range of 77.0% to 100.0% with a CV of 3.39%－10.05%. The results indicated that this developed method was characteristics of high sensitivity and accuracy as wall as low detection limit, which could meet the requirement of MRL determination.

Key words: gibberellin, residue, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)

CLC Number:

TS207.3

ZHAO Ying-bo1，ZHOU Yan-ming1,*，XIN Xue1,2，WANG Yan-song3. Determination of Gibberellin Residue in Vegetables and Fruits by HPLC-MS/MS[J]. FOOD SCIENCE, 2011, 32(6 ): 209-212 https://doi.org/10.7506/spkx1002-6630-201106048

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Determination of Gibberellin Residue in Vegetables and Fruits by HPLC-MS/MS

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