Abstract:

Cellulase hydrolysis, microwave-assisted extraction and cellulase hydrolysis followed by microwave-assisted extraction were investigated individually for the optimal extraction of hesperidin from“ Bingtang” sweet orange (Citrus sinensis L. Osbeck) peels. Results showed that the optimal hesperidin extraction was based on 90 min cellulase hydrolysis at 45 ℃ and pH 5.5 with an enzyme dose of 3% (calculated on the basis of dry weight of material) followed by extraction with 80% ethanol at a solid/ liquid ratio of 1:50 under the assistance of microwave treatment for 25 s at 560 W power. In three replicate extractions using the above process, the average yield of hesperidin was as high as 6.130%, and the relative standard deviation was 0.28%, which demonstrates the applicability of the process for extracting hesperidin from “Bingtang” sweet orange peels.

Key words: ＂Bingtang＂ sweet orange, hesperidin, cellulose, microwave-assisted extraction