Identification of IgE-binding Epitopes on β-lactoglobulin

doi:10.7506/spkx1002-6630-201015041

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (15): 190-193.doi: 10.7506/spkx1002-6630-201015041

• Bioengineering • Previous Articles Next Articles

Identification of IgE-binding Epitopes on β-lactoglobulin

CONG Yan-jun1，REN Fa-zheng2,*，YUN Zhan-you3

1. School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100048, China；
2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China；
3. Inner Mongolia Yili Industrial Group Co. Ltd., Hohhot 010080, China

Received:2009-11-24 Online:2010-08-15 Published:2010-12-29
Contact: REN Fa-zheng2,*， E-mail:renfazheng@263.net

Abstract

Abstract:

A series of overlapping decapeptides (offset by five amino acids) were synthesized on the basis of the amino acid sequence of β-lactoglobulin. IgE-binding epitopes were identified using individual sera from cow milk allergic patients. In addition, we also identified critical amino acids (AAs) for IgE binding. The results showed that four IgE-binding epitopes were identified, which located in the sequences of AA 17 － 31, AA 72 － 86, AA 92 － 106 and AA 152 － 166, respectively. Threonine, methionine and aspartic acid were the critical amino acids for allergenicity of β-lactoglobulin.

Key words: cow milk, β-lactoglobulin, IgE, epitope

CLC Number:

TS252.1

CONG Yan-jun1，REN Fa-zheng2,*，YUN Zhan-you3. Identification of IgE-binding Epitopes on β-lactoglobulin[J]. FOOD SCIENCE, 2010, 31(15): 190-193.

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Identification of IgE-binding Epitopes on β-lactoglobulin

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