Abstract:

In order to construct a set of technical systems for protein profile analysis of skeletal muscle of fugu (Takifugu rubripes), total proteins extracted from fugu skeletal muscle were separated using high resolution two dimensional polyacrylamide gel electrophoresis and developed by silver staining in polyacrylamide gel for computer assisted analysis of electrophoresis patterns using software IMAGEMASTERTM. Subsequently, matrix assisted laser desorption ionization time of flight mass spectrometry (Maldi TOF MS ) was applied for identifying the major protein spots on the 2D profile. Of the 112 protein spots detected in a silver-stained 2D polyacrylamide gel, 33 were analyzed using Maldi TOF MS, and 21 were identified using MASCOT against takifugu or zebrafish data in NCBI nr, MSDB, and UniProtKB/TrEMBL, which included structural proteins such as tropomyosins, enzymes or proteins involved in metabolism and signal transduction and apoptosis etc.

Key words: Takifugu rubripes, skeletal muscle, 2D electrophoresis, Maldi TOF MS, proteomics