Purification and Antioxidant Activity of Peptides Derived from Egg White Proteins

doi:10.7506/spkx1002-6630-200915005

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (15): 30-32.doi: 10.7506/spkx1002-6630-200915005

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Purification and Antioxidant Activity of Peptides Derived from Egg White Proteins

ZHOU Guo-yi，CHENG Jing，CHEN Dong-liang*，WANG A-jing，YANG Guo-yan，JI Jin-lin

Application Research Centre for Peptide Substance, Wuhan Tallyho Biological Product Co. Ltd., China
Polypeptide Group, Wuhan 430023, China

Received:2008-12-03 Revised:2009-06-08 Online:2009-08-01 Published:2010-12-29
Contact: CHEN Dong-liang*， E-mail:tallyho@public.wh.hb.cn

Abstract

Abstract:

The peptides derived from egg white proteins were fractionated on Sephadex G-25 gel filtration column and the fraction with the highest DPPH· radical scavenging activity was purified on Sephadex G-15 gel filtration column. A total of 7 fractions were obtained by Sephadex G-25 gel filtration chromatography and fraction 3 showed the highest antioxidant activity with a DPPH· radical scavenging rate of 63.80% at 3 mg/ml. Fraction 3 was further separated into 4 subfractions, among which, subfraction 3-1 and 3-2 showed higher DPPH radical scavenging activities and the DPPH radical scavenging rates at 3 mg/ml were 84.02% and 81.17%, respectively. According to RP-HPLC chromatogram subfraction 3-1 had a high homogeneous composition.

Key words: peptides derived from egg white proteins, purification, antioxidant activity

CLC Number:

Q516

ZHOU Guo-yi，CHENG Jing，CHEN Dong-liang*，WANG A-jing，YANG Guo-yan，JI Jin-lin. Purification and Antioxidant Activity of Peptides Derived from Egg White Proteins[J]. FOOD SCIENCE, 2009, 30(15): 30-32.

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Purification and Antioxidant Activity of Peptides Derived from Egg White Proteins

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