FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (13): 22-24.

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Isolation and Purification of Water-soluble Polysaccharide from Common Buckwheat (Fagopyum esculentum) and Determination of Its Molecular Weight

XU Wen-tao1,2，ZHANG Fang-fang2，LUO Yun-bo2，WANG Ying2，HUANG Kun-lun1,2,*   

  1. 1. Laboratory of Food Safety and Molecular Detection, College of Food Science and Nutritional Engineering, China
    Agricultural University, Beijing 100083, China；2. Supervision, Inspection and Testing Center of Genetically Modified Food
    Safety, Ministry of Agriculture, Beijing 100083, China
  • Received:2008-04-09 Revised:2008-12-03 Online:2009-07-01 Published:2010-12-29
  • Contact: XU Wen-tao E-mail:xuwentao1111@sina.com;cauxwt@yahoo.cn

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Abstract:

A water-soluble polysaccharide from Fagopyum esculentum (FEP) was obtained by boiling water extraction and ethanol precipitation, followed by consecutive purification on DEAE-Sepharose Fast Flow column and Sephacryl S-400 HR column, and was subjected to freeze-drying. The elution profile of FEP on Sephacryl S-400 HR column presented only a single symmetrical peak, indicating that the FEP was purified to a homogeneous polysaccharide. The polysaccharide content in dried FEP powder was 98.5% determined by phenol-sulfuric acid assay, and no protein components were detected in the powder. The molecular weight of the FEP was calculated as 1720442 D, according to the calibration curve of standard dextrans.

Key words: Fagopyum esculentum, polysaccharide, isolation and purification, determination of molecular weight

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