FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (12): 165-167.doi: 10.7506/spkx1002-6630-200912034

Previous Articles     Next Articles

Simultaneous RP-HPLC Determination of Lactic Acid and Methyl Lactate

JIANG Shao-tong,LIU Mo,CHEN Xiao-yan,ZHENG Zhi   

  1. (School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
    Abstract
  • Received:2008-08-29 Revised:2008-11-22 Online:2009-06-15 Published:2010-12-29
  • Contact: JIANG Shao-tong, E-mail:jiangst@hfut.edu.cn

Abstract:

A reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of lactic acid and methyl lactate. The HPLC was equipped with Purospher STAR C18 reversed-phase column and diode array detector. The mobile phase was composed of 0.005 mol/L H2SO4 solution and chromatographically pure methanol (9:1, V/V) with the flow rate of 1.0 ml/min. The injection volume was 20 μl, and the detection wavelength was set as 210 nm. This method is accurate, simple, rapid and reproducible.

Key words: lactic acid, methyl lactate, reverse phase-high performance liquid chromatographic (RP-HPLC)

CLC Number: