Abstract:

An ultra performance liquid chromatography (UPLC) method for determination of clopidol in chicken was developed. The clopidol residue in chicken was extracted by acetonitrile, defatted by n-hexane, purified by alumina B cartridges, analyzed by HPLC with UV detector at 267 nm and acetonitrile-water (10:90, V/V) solvent as mobile phase, and quantified by using external standard calibration curve. The results showed that there was a nice linear relationship (r ＞ 0.999) when clopidol concentration was in the range of 10 to 1000 μg/L. The average recoveries at three addition levels (10, 50 and 100 μg/kg) were 60.5% to 94.6%. The within-day and inter-day variation coefficients were between 4.2% and 10.2% and between 6.6% and 12.7%, respectively. The limit of detection was 5 μg/kg, and the limit of quantification was 15 μg/kg.

Key words: clopidol, ultra performance liquid chromatography (UPLC), chicken, residue