FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (8): 204-206.doi: 10.7506/spkx1002-6630-200908043

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Catalytic Kinetic Spectrophotometric Determination of Ascorbic Acid

CHEN Yan-qing1,2 ZENG Gui-sheng2 NI Yong-nian1,*   

  1. (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
    2. College of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang 330063, China)
  • Received:2008-05-26 Revised:2008-06-30 Online:2009-04-15 Published:2010-12-29
  • Contact: NI Yong-nian1,* E-mail:ynni@ncu.edu.cn

Abstract:

A new catalytic kinetic spectrophotometric method was proposed for determining trace ascorbic acid based on the catalytic effect of ascorbic acid on the oxidation of potassium dichromate with rhodamin B in nitrate acid solution. Good linearity is obtained in the concentration range of 0.20 to 4.0 μg/ml of ascorbic acid, and the detection limit is 0.050 μg/ml. The method is used to determine trace ascorbic acid in fruits and juice with satisfactory results.

Key words: ascorbic acid, catalytic kinetic spectorphotometry, rhodamine B, potassium dichromate

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