FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (5): 214-216.doi: 10.7506/spkx1002-6630-200905050

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Study on Aqueous Two-phase Extraction of Xylanase

SU Yu-chun,CHEN Guang*,Bai Jing,HAN Wei-wei,WANG Zhong-xing   

  1. (College of Life Science, Jilin Agricultural University, Changchun 130118, China)
  • Received:2008-05-26 Revised:2008-09-04 Online:2009-03-01 Published:2010-12-29
  • Contact: CHEN Guang*, E-mail:cg61@163.com

Abstract:

Orthogonal tests were designed to optimize the aqueous two-phase extraction process of xylanase from fermentation broth of Aspergillus niger AS3.4039 in this study. The results indicated that the optimum concentrations of PEG 4000, K2HPO4 and NaCl are 19%, 10% and 0 (W/W), respectively. Under these conditions, the separation factor (K) and xylanase yield in top phase (Yt) are 6.23 and 88.67% respectively, and the purification effect is satisfying.

Key words: aqueous two-phase extraction, xylanase, purification

CLC Number: