Abstract:

Objective: To optimize process conditions of separation and purification of total flavonoids from Lycium ruthenicum Murr. with macroreticular resin. Methods: Siginal factor and orthogonal tests were done. Results: Using AB-8 resin to adsorb and purify total flavonoids from Lycium ruthenicum Murr, the optimal purification conditions are as follows: concentration of sample solution 0.200 mg/ml,velocity of sample solution 2 ml/min, pH 5, velocity of 95% ethanol taken as eluent 4 ml/min, and volume of 95% ethanol 5 BV (bed volume). The saturated adsorption capacity of AB-8 is 0.64691 mg/g resin, and after used 9 times repeatedly, adsorption capacity of AB-8 is still good. With these paramaters, the purity of product is 28.0%. Conclusion: AB-8 resin is effective in the adsorption and purification of the total flavonoids form Lycium ruthenicum Murr. with good repetition and stability. Therefore, the above technice is suitable to industrial production.

Key words: Lycium ruthenicum Murr., total flavonoids, macroreticular resin, adsorption and purification