FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (1): 173-176.doi: 10.7506/spkx1002-6630-200901041

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Purification of Polyphenol Oxidase from Lotus Roots

HU Wan-feng,ZHAO Hong-lei,WAN Qiong-hong,LI Chun-mei*,CHEN Mei-hong   

  1. (College of Food Science and Technology , Huazhong Agricultural University, Wuhan 430070, China)
  • Received:2007-12-09 Revised:2008-05-20 Online:2009-01-01 Published:2010-12-29
  • Contact: LI Chun-mei E-mail:lichmyl@mail.hzau.edu.cn

Abstract:

The polyphenol oxidase (PPO) of lotus root was extracted and purified by four steps: ammonium sulfate fractionation with saturation 30% and 80%, and DEAE-Sephadex A-50 and Sephadex G-75 column chromatography in turn. SDS-PAGE analysis showed that the purified PPO is a single polypeptide with a molecular weight of about 21.0 kD. The final purification fold and the yield of PPO are 103 and 0.38 %, respectively.

Key words: lotus root, polyphenol oxidase, purification

CLC Number: