Abstract: Purpose ：To prepare high fructose corn syrup F90 (with a fructose content of 90%) from F42 (with a fructose content of 42%) by simulated moving bed chromatography (SMB). Methods ：The effects of resin type, feeding volume and rate, corn syrup concentration and operating temperature on separation degree were investigated by single-column pulse experiments. The theoretical SMB parameters were identified by the safety factor method and experimentally modified to be the optimal parameters. Results：The theoretically stimulated and experimental results showed good agreement. The optimal SMB parameters were switching time 469 s, feeding rate 1.574 L/h, elution solution flow rate 0.325 L/h, extraction solution flow rate 0.902 L/h, raffinate flow rate 0.951 L/h, and cycling flow rate 1.897 L/h. The yield and purity in the final product were 80.32% and 99.91% for fructose and 90.13% and 92.34% for glucose, respectively,

Key words: simulated moving bed, safety factor method, parameter design, high pure fructose