Ultrasonic-assisted Extraction and Purification of Water-soluble Polysaccharides from Soybean Dregs

doi:10.7506/spkx1002-6630-201106031

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (6 ): 139-142.doi: 10.7506/spkx1002-6630-201106031

• Processing Technology • Previous Articles Next Articles

Ultrasonic-assisted Extraction and Purification of Water-soluble Polysaccharides from Soybean Dregs

CHEN Hong，ZHANG Bo，LIU Xiu-qi，LI Hong，WANG Da-wei*

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China

Received:2010-07-11 Revised:2011-02-10 Online:2011-03-25 Published:2011-03-03
Contact: CHEN Hong E-mail:chenhong216@yahoo.com.cn

Abstract

Abstract: Skimmed extruded soybean dregs were used as the raw material to prepare water-soluble polysaccharides through the steps of ultrasonic-assisted extraction and purification by Sevag s method. The optimal ultrasonic-assisted extraction parameters were found to be extraction pH of 4.5, temperature of 90 ℃, material/liquid ratio of 1:20 (g/mL), ultrasonic power of 200 W, and ultrasonic extraction time of 40 min. Under these optimal extraction conditions, the extraction rate of water-soluble soybean polysaccharides was up to 8.82%. The purification of crude polysaccharides was achieved by treating with a Sevag reagent composed of CHCl3 and n-butanol (3:1, V/V) 3 times. The recovery rate of purified polysaccharides was up to 60.30%.

Key words: skimmed extruded soybean dregs, water-soluble soybean polysaccharide, ultrasonic extraction, purification

CLC Number:

TS214.2

CHEN Hong，ZHANG Bo，LIU Xiu-qi，LI Hong，WANG Da-wei*. Ultrasonic-assisted Extraction and Purification of Water-soluble Polysaccharides from Soybean Dregs[J]. FOOD SCIENCE, 2011, 32(6 ): 139-142.

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Ultrasonic-assisted Extraction and Purification of Water-soluble Polysaccharides from Soybean Dregs

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