Isolation, Purification and Antioxidant Activity Evaluation of Water Soluble Protein from Giant Mealworm Beetle (Zophobas morio) Larvae

FOOD SCIENCE ›› 0, Vol. ›› Issue (): 30-33.

Isolation, Purification and Antioxidant Activity Evaluation of Water Soluble Protein from Giant Mealworm Beetle (Zophobas morio) Larvae

Zhang Jian-xin

Received:2010-11-10 Revised:2011-07-21 Online:2011-09-25 Published:2011-09-24
Contact: Zhang Jian-xin E-mail:zhangjx59@foxmail.com

Abstract

Abstract: Objective: To isolate and purify water soluble protein from giant mealworm beetle (Zophobas morio) larvae and evaluate its antioxidant activity. Methods: The water soluble protein in giant mealworm beetle larvae was extracted with pH 7.4 phosphate buffer, purified by ammonium sulfate precipitation, and fractionated by Sephadex G-100 column chromatography to obtain fractions P1, P2 and P3. Fraction P2 was further fractionated by DEAE-Sepharose FF column chromatography into subfractions P2M1, P2M2 and P2M3. The antioxidant activities of each fraction and each subfraction were investigated by DPPH and hydroxyl free radical scavenging assays. Results: Subfraction P2M2 presented the highest DPPH and hydroxyl free radical scavenging rates, which were 99.42% and 55.11%, respectively.

Key words: giant mealworm beetle, protein, isolation and purification, antioxidant activity

Zhang Jian-xin. Isolation, Purification and Antioxidant Activity Evaluation of Water Soluble Protein from Giant Mealworm Beetle (Zophobas morio) Larvae[J]. FOOD SCIENCE, 0, (): 30-33.

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Isolation, Purification and Antioxidant Activity Evaluation of Water Soluble Protein from Giant Mealworm Beetle (Zophobas morio) Larvae

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