Extraction, Purification and Antioxidant Activity in vitro of Protein Fractions from Dioscorea opposita Thunb. cv. Tiegun

Abstract

Abstract: Crude protein extracted from Dioscorea opposita Thunb.cv. Tiegun by saturated ammonium sulfate precipitation was purified/fractionized by DEAE-52 ion exchange chromatography, and two fractions were obtained and named as PDOT-1 and PDOT-2, respectively. Further Sephadex G-100 chromatography revealed that both fractions were composed of a single component. The non-reducing SDS-PAGE pattern of PDOT-1 displayed three protein bands with a molecular mass of respectively 20, 31 kD and 50 kD and only one 66 kD protein band was observed in that of PDOT-2. It was found that the crude protein, PDOT-1 and PDOT-2 had obvious radical scavenging activities on DPPH, superoxide anion and hydroxyl free radicals as well as strong ferrous ion-chelating capacity. We concluded that proteins from Dioscorea opposita Thunb.cv. Tiegun have antioxidant activity and deserve to be studied in depth as a potential anoxidant or anti-aging drug.

Key words: Dioscorea opposita Thunb.cv. Tiegun, protein, extraction and purification, antioxidant activity

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Extraction, Purification and Antioxidant Activity in vitro of Protein Fractions from Dioscorea opposita Thunb. cv. Tiegun

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