FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (4 ): 230-232.doi: 10.7506/spkx1002-6300-201004053

• Analysis & Detection • Previous Articles     Next Articles

High-performance Liquid Chromatography Coupled with Tandem Mass Spectrometric Determination of Steroid Hormone Multi-Residues in Pork

NIU Jin-yang,SUN Huan,LI Ying-ying   

  1. China Meat Research Center, Beijing 100068, China
  • Received:2009-10-30 Online:2010-02-15 Published:2010-12-29

Abstract:

A method using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was proposed for the determination of steroid hormone multi-residues in pork. Analytes were extracted from samples with methanol and cleaned up using a solid-phase extraction cartridge prior to HPLC-MS/MS analysis. The proposed method exhibited a good linearity for 10 steroid hormones over the range of 0.5-100μg/kg and a limit of detection ranging from 0.2 to 2μg/kg. Mean recoveries for 10 steroid hormones in blank pork samples spiked at low, medium and high concentrations were between 71% and 88%, with relative standard deviations below 10%. The method is characteristic of rapidity, simplicity, good selectivity and high sensitivity, and therefore has a great popularization potential.

Key words: steroid hormone, pork, HPLC/MS-MS

CLC Number: