Abstract:

Objectives: To explore regulation effects and mechanisms of immune colostrum on nutrition and metabolism in acute infectious diarrhea diseases. Methods: Thirty-two healthy adult SD rats with half of female and male were randomly divided into a control group (GC), a model group (GM), a group protected with normal colostrum (GNC) and a group protected with immune colostrum (GIC). Rats in GNC and GIC were orally administered with normal and immune colostrum at a dose of 1 ml/100 g body weight once a day for 12 days, respectively. Rats in GC and GM were administered saline using the same methods as controls. At 5 and 10 d post-experiment, the rats in GM, GNC and GIC, respectively, were orally infected with mixed enteroinvasive strains containing 1×108/ml of E. coli and Salmonella typhi at a dose of 1 ml/100g body weight once. Results: Free fatty acid (FFA) level in plasma of GM was obviously lower than that of GC; in contrast, FFA level in plasma of GNC was significantly higher than that of GC. Meanwhile, glucose in plasma of both GM and GNC was obviously higher than that of GC. However, no significant difference was observed between GIC and GC. At the same time, a significantly elevated insulin level in serum of GNC was detected compared with GM and GNC. The sIgA level exhibited an increase in GM and a reduction in GNC compared with GC, but no obvious difference was revealed between GIC and GC. Conclusion: Immune colostrum may attenuate stimulations from diarrhea pathogen bacteria on intestinal mucosa immune system to regulate and maintain a relative stabilization of Glu or FFA content and effectively protect anti-infectious diarrhea in rats.

Key words: immune colostrums, rat, diarrhea, insulin, free fatty acid, glucose, sIgA