Synthesis and Identification of Corticosteroids Immunogens

doi:10.7506/spkx1002-6630-200917035

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (17 ): 149-154.doi: 10.7506/spkx1002-6630-200917035

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Synthesis and Identification of Corticosteroids Immunogens

HU Yong-ming，YUAN Yuan，LIU Li-qiang，CHEN Wei，PENG Chi-fang，XU Chuan-lai*

School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Received:2009-07-08 Online:2009-09-01 Published:2014-04-14
Contact: XU Chuan-lai E-mail:xcl@jiangnan.edu.cn

Abstract

Abstract:

Three haptens such as dexamethasone-21-HS (DEX-HS), betamethasone-21-HS (BET-HS) and flumethasone21- HS (FLM-HS) prepared using N-hydroxysuccinimide (NHS) were conjugated separately with bovine serum albumin (BSA) and ovalbumin to prepare immunogens and coating antigens. The conjugation ratios were estimated to be DEX-BSA:11, BET-BSA: 23 and FLM-BSA:17, respectively using UV spectrophotometry.

Key words: corticosteroids, immunogen, synthesis, identification

CLC Number:

S852.4.3

HU Yong-ming，YUAN Yuan，LIU Li-qiang，CHEN Wei，PENG Chi-fang，XU Chuan-lai*. Synthesis and Identification of Corticosteroids Immunogens[J]. FOOD SCIENCE, 2009, 30(17 ): 149-154.

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Synthesis and Identification of Corticosteroids Immunogens

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