Abstract:

This study investigated the hypoglycemic effect of polysaccharide extracted from fruit body of Pleurotus citrinopileats on diabetic mice to provide theoretical reference to the development of functional food or natural drug can reduce blood glucose. Mouse models of diabetes were constructed by administering alloxan to mice through intraperitoneal injection. Pleurotus citrinopileats polysaccharide at doses of 100 and 400 mg/kg bw was given to the diabetic mice by intragastric administration for 30 d, and low- and high-dose polysaccharide treated groups were obtained. At the same time, 100 mg/kg bw of glybenzoylamide was given to the diabetic mice which were recognized as drug-treated group, and normal control group and Pleurotus citrinopileats polysaccharide control group given dose of 400 mg/kg bw were also constructed. The fasting blood glucose level and glucose tolerance capacity were determined at appointed time and the contents of saccharified hemoglobin and liver glycogen in sacrificed mice were assayed. After the diabetic mice were treated for 30 d with Pleurotus citrinopileats polysaccharide, the clinical symptoms of diabetes were obviously improved when compared with those of diabetes model group, the fasting blood sugar level and area under the blood glucose curve were significantly decreased (P < 0.01), and the contents of GHB and liver glycogen came back to the normal level. Moreover no significant changes in blood glucose index were found in Pleurotus citrinopileats polysaccharide control group in comparison with normal control group.

Key words: Pleurotus citrinopileats polysaccharide, hypoglycemic effect, diabetes