Preparative High-speed Counter-current Chromatography for Preparation of Ricinine from Seeds of Ricinus cammunis L. (Caster Bean)

doi:10.7506/spkx1002-6630-200914026

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (14 ): 149-152.doi: 10.7506/spkx1002-6630-200914026

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Preparative High-speed Counter-current Chromatography for Preparation of Ricinine from Seeds of Ricinus cammunis L. (Caster Bean)

CHEN Ya-fang1，SHI Bo1,*，CHENG Yong-qiang2，WANG Shao-yun1，LIANG Ping1

1. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China；
2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Received:2009-03-01 Online:2009-07-15 Published:2010-12-29
Contact: SHI Bo1,*， E-mail:shibo@mail.caas.net.cn

Abstract

Abstract:

The preparative high-speed counter-current chromatography (HSCCC) method was applied to isolate and purify ricinine from the crude extract of Ricinus cammunis L. seeds, and the purity of purification product was measured by HPLC and its structure was analyzed by MS and NMR. After being defatted using a mixture of petroleum ether and ethyl ether (2:1, V/V) twice, caster bean seeds without shell was subjected to ricinine extraction using 95% ethanol in a Soxhlet extractor followed by vacuum concentration and freeze drying. The preparative HSCCC was performed using a two-phase solvent system composed of chloroform, methanol and water (2:1:1, V/V). A total amount of 70 mg of ricinine with over 99.62% purity was obtained from 100 mg of the crude extract. Ricinine was identified as the main component in the crude extract by MS and NMR. The preparative HSCCC is suitable for large-scale preparation of ricinine with high purity.

Key words: caster bean, ricinine, two-phase solvent system, high-speed counter-current chromatography (HSCCC), purification

CLC Number:

TS222.1

CHEN Ya-fang1，SHI Bo1,*，CHENG Yong-qiang2，WANG Shao-yun1，LIANG Ping1. Preparative High-speed Counter-current Chromatography for Preparation of Ricinine from Seeds of Ricinus cammunis L. (Caster Bean)[J]. FOOD SCIENCE, 2009, 30(14 ): 149-152.

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Preparative High-speed Counter-current Chromatography for Preparation of Ricinine from Seeds of Ricinus cammunis L. (Caster Bean)

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