FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (4): 221-224.
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XING Fang-Fang, YIN Yu-Long, HUANG Rui-Lin, KONG Xiang-Feng, LI Tie-Jun, TANG Zhi-Ru, ZHANG You-Ming
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Abstract: After cloning into the expression vector pET28a, the lactoferricin B gene was transformed into BL-21 E.coli prokaryotic expression system. By selecting expression temperature and IPTG concentration, lactoferricin B gene was expressed at high level in prokaryotic cells. The expression of lactoferricin B was detected by tricine-SDS-PAGE protein electrophoresis and functional test, and it was proved to be the lactoferricin B. The result also showed that the gene of lactoferricin B can be highly expressed in prokaryotic expression system, and the expression product accounts for about 21% of total bacterial proteins with biology activity.
Key words: lactoferricin B gene, prokaryotic expression system, Tricine-SDS-PAGE, functional test
XING Fang-Fang, YIN Yu-Long, HUANG Rui-Lin, KONG Xiang-Feng, LI Tie-Jun, TANG Zhi-Ru, ZHANG You-Ming. Expression of Recombinant Lactoferricin B in E.coli[J]. FOOD SCIENCE, 2008, 29(4): 221-224.
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