Abstract: Effects of ball-milling treatment on particle size distribution, specific surface area, calcium extraction rate and soluble protein content of sliver carp bone powder were studied. Results showed that the particle size increases followed by a decrease with ball-milling time prolonging. The average particle size (Dav) and median particle size (D50) of sliver carp bone powder are minimum by ball-milling treatment for 30 h, 9.33 μm and 7.05 μm, respectively. Particle size increases by more than 30 h ball- milling treatment due to second aggregation. Specific surface area, calcium extraction rate and soluble protein content decrease followed by an increase with ball-milling time prolonging. Maximum value of specific surface area, calcium extraction rate and soluble protein content could be obtained 30 h ball-milling treatment, as 94314 m2/m3, 13.96 % and 42.70 mg/g, respectively. The values decrease slightly when ball-milling time was more than 30 h.

Key words: ball-milling, fish bone powder, particle size distribution, specific surface area, calcium extraction rate