Abstract: Crude product of ε-poly-L-lysine by producted by 5-L fermenter; The antibacterial activities of ε-poly-L-lysine on common bacteria were determined by Oxford cup assay and dilution-plate methods. The effect of temperature on antibacte- rial activity of ε-poly-L-lysine, the activity of combination of it with acetic acid or glycine, and its application in the preservation of sliver carp surimi were studied. The results showed that ε-poly-L-lysine has a wide antibacterial spectrum and can inhibit gram- negative, gram-postive bacteria and fungi; ε-Poly-L-lysine is good themal stabitity when temperature is 80, 100, 121℃; The antibacterial activity of ε-poly-L-lysine is enhanced by combination with acetic acid or glycine; ε-Poly-L-lysine has good preservation effect for sliver carp surimi and is an effective natural food preservative.

Key words: &epsilon, -poly-L-lysine； antibacterial activity； natural food preservative