Study on Preparation of Soybean Peptide from Soybean Protein by Alkaline Protease Hydrolysis on Quadratic Ratation Combitination Genernal Design

Abstract

Abstract: Soybean protein was hydrolyzed by alkaline protease to produce soybean peptide.Extent of reaction was expressed in terms of degree of hydrolysis.By single factor and quadratic ratation combitination genernal design hydrolysis condition model was established and the optimum hydrolyzing conditions are temperature 55 ℃,pH value 9.5,enzyme concentration was established 6 %,substrate concentration 2 %,and hydrolyzing time 1.0 hours.On these conditions,nitrogen solubility index(NSI) and degree of hydrolysis(DH) of hydrolysate are respectively 93.16 % and 92.27 %.

Key words: protease, soybean protein, soybean peptide, quadratic ratation combitination genernal design

CONG Jian-Min. Study on Preparation of Soybean Peptide from Soybean Protein by Alkaline Protease Hydrolysis on Quadratic Ratation Combitination Genernal Design[J]. FOOD SCIENCE, 2008, 29(5): 319-323 https://doi.org/

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Study on Preparation of Soybean Peptide from Soybean Protein by Alkaline Protease Hydrolysis on Quadratic Ratation Combitination Genernal Design

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