FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (1): 25-30.doi: 10.7506/spkx1002-6630-201201004

• Basic Research • Previous Articles     Next Articles

Effect of Lipid Oxidation on Protein Structure

ZHANG Yin-liang,AN Qiao-yun,YANG Hui   

  1. School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Chinagzip,
  • Received:2011-02-14 Revised:2011-12-27 Online:2012-01-15 Published:2012-01-12
  • Contact: Zhang Yin-Liang E-mail:zhyinliang@163.com

Abstract:

This paper deals with the effect of lipid oxidation on protein structure. Oxidized lipid products were prepared by oxidizing linoleic acid under different conditions and allowed to react with bovine serum albumin (BSA) under the same conditions (25 ℃, 4 h). Changes in various properties of BSA were determined during the reaction with oxidized lipid products. The results showed that oxidized lipid products revealed a decrease in thiobarbituric acid value (TBA) after reaction with BSA, while the oxidation value (carbonyl group content) and surface hydrophobicity of BSA increased. Meanwhile, the maximum emission wavelength of the protein showed a red shift and the secondary structure varied obviously. Therefore, lipid oxidation has a significant impact on protein structure.

Key words: lipid oxidation, protein, TBA value, protein oxidation value, surface hydrophobicity, secondary structure

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