Isolation, Purification and Characterization of Water-soluble Polysaccharides from Ampelopsis grossedentata

Abstract

Abstract: Polysaccharide from Ampelopsis grossedentata (AGP) was obtained after being extracted with hot water, precipitated with alcohol and removed free protein with Sevag method. AGP was isolated and purified by DEAE-Sephadex A-25 and Sephadex G-200 column chromatography, two pure polysaccharides (AGP-3 and AGP-4) were gained. The results of HPLC and Sephadex G-200 column chromatography illustrated that both AGP-3 and AGP-4 were protein-bond polysaccharids with comparatively homogenous molecular weights. Also, the two polysaccharids were colored by coomassie brilliant blue G-250 and carbazole reaction.

Key words: Ampelopsis grossedentata, polysaccharide, isolation, purification, characterization

LUO Zu-You, CHENG Chao, LI Wei, WU Mou-Cheng. Isolation, Purification and Characterization of Water-soluble Polysaccharides from Ampelopsis grossedentata[J]. FOOD SCIENCE, 2007, 28(1): 151-154.

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Isolation, Purification and Characterization of Water-soluble Polysaccharides from Ampelopsis grossedentata

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