FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (1): 209-212.

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Study on Proteinse Activity and Protein Content in Corn during Germination

 HAN  Chun-Ran   

  1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China
  • Online:2007-01-15 Published:2011-12-31

Abstract: Factors influencing the activity of proteinase in corn during germination were studied, and the molecular weight of soluble protein after corn germinating was determined by SDS-PAGE. The results showed that the proteinase activity is affected by moisture content, temperature, light and gibberellin acid (GA). The maximum activity of proteinase was obtained on the following conditions, moisture content 35%, temperature 30℃, gibberellin acid 30mg/L, natural light; after germination for 6 days, there were two obvious protein bands with the molecular weight lower than 20kD, the coleculare weight of band band c was 19.2kD and 18.2kD, respectively.

Key words: corn, germination, proteinase, molecular weight