FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (1): 255-257.

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Determination of Chlorogenic Acid in Eucommia Ulmoides Olive Leaves by Reversed-Phase High Performance Liquid Chromatography

 WAN  Chun-Hua, NI  Yong-Nian, LONG  Zhou-Xiong, HU  Hai-Shan, YAN  Bing   

  1. 1.Department of Chemistry, Nanchang University, Nanchang 330029, China; 2.Jiangxi Institute of Analysing and Testing , Nanchang 330029, China
  • Online:2007-01-15 Published:2011-12-31

Abstract: The conditions for the extracting chlorogenic acid in the Eucommia Ulmoides Olive leaves were optimized by the orthogonal test as follows: continuous extraction by 30% methanol for 20 minutes; determined by RP-HPLC. The chlorogenic acid was seperated on kromasil C18 column, with CH3OH~0.02%H3PO4 (22:78) at a flow-rate of 1.0ml/min and detected at UV 327nm.The calibration curve was linear in the range of 9.68~242μg/ml (r=0.9999). This method is simple, rapid and accurate with good separation, and the recoveries are above 95.9%.

Key words: Eucommia Ulmoides Olive leaves, chlorogenic acid, reversed-phase high performance liquid chromatography (RP-HPLC), orthogonal test