Abstract: The extraction and purification technology of the water-soluble polysaccharide from Fructus Corni was studied in this paper. Several factors of affecting extraction rate such as extraction temperature, extraction times and the ratio of sample to water (W:V) were studied. The results showed that the extraction temperature is the major factor to affect the polysaccharide extraction rate. By uniform design test the optimum extraction conditions are as follows: extraction temperature 100 ℃, extraction time 180 min and the ratio of sample to water 1:36. The extraction rate reaches 199.36 mg/g under the optimal extraction conditions. The crude polysaccharide was purified by ethanol sedimentation, dialysis, DE-52 chromatography. The purified Fructus Corni polysaccharide was examined with ultraviolet spectral analysis without any protein, nuclear acid. It showed that size is obtained homogeneous single composition polysaccharide of molecule by agarose gel electrophoresis shown in the single area.

Key words: Fructus Corni polysaccharide, uniform design, extraction, purification