Research on Extraction and Purification of Lutein from Brassic oleraced var. acephala DC.

Abstract

Abstract: The aim of this study is to determine the best conditions extracting lutein from Brassic oleraced var. acephala DC., based on its dried powder being soaked in THF(10:1) and saponified through 10% KOH-C2H5OH (6.5:1). Raw lutein was purified through recrystallization and its purity was measured with HPLC. The results showed that under the circumstances of 40Hz ultrasonic wave, the extracted lutein rate reached 3289.42 μg/100 g with the extracted temperature 30 ℃ and the extracted time 20 min. The purity of lutein in free state reached 88.13%.

Key words: lutein, Brassic oleraced var. acephala DC., ultrasonic wave, extraction, purification

WANG Xiang-Dong, ZHAO Guo-Jian. Research on Extraction and Purification of Lutein from Brassic oleraced var. acephala DC.[J]. FOOD SCIENCE, 2007, 28(7): 145-148.

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Research on Extraction and Purification of Lutein from Brassic oleraced var. acephala DC.

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