Purification of Mogroside from Fructus Momordicae

Abstract

Abstract: Component A was obtained from Fructus Momordicae extract by tandem AB-8/ADS-7 column chromatography followed by thin layer chromatography (TLC) using n-buttoanol-CH3COOH-water (4:1:2) as the development solvent. The component was separated into fractions A1, A2 and A3 by preparative high performance chromatography (HPLC) on ODS column (Ф 20 mm ×250 mm) using 30% aqueous methanol as the mobile phase at a flow rate of 8 mL/min, in which the detection wavelength was set as 210 nm. Fraction A2 was identified by HPLC as mogroside. Fractions A1 and A3 were unknown compounds.

Key words: Fructus Momordicae, mogroside, purification

CLC Number:

TS201.1

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Purification of Mogroside from Fructus Momordicae

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