Abstract: Besides contents of element Co and element I, the effect of initial pH in medium on accumulation of vitamin E (VE) for wheat embryo callus was studied. The mathematical models were established to describe growth kinetics and accumulation of vitamin E for wheat embryo callus cells. Based on the maximum accumulation of secondary metabolites vitamin E as an objective, the best combination of contents of Co and I with initial pH in medium was determined by orthogonal test. The results showed that the production of vitamin E in N6 medium is maxmium at the concentrations of CoCl2 0.025 mg/L and KI 0.80 mg/L as well as initial pH 5.8. The fitting degree of kinetic model of VE accumulation is 98.11%, and the fitting degree of kinetic model of cell growth is 99.40%, with good nonlinear relationship. The beneficial elements both Co and I affect accumulation of VE in medium for wheat germ callus, and the effect of the former is remarkabler than that of the later.

Key words: wheat embryo, accumulation of vitamin E, beneficial element, nonlinear models