Isolation and Purification of Polysaccharide from Seeds of Plantago asiatica L. and Analysis of Its Monosaccharide Compositions

Abstract

Abstract: In this study, polysaccharides were isolated with boiling water from Plantago asiatica L., and then the protein in the isolates was removed by Sevag method. Successively, the polysaccharides were further purified using the AKTA purifier 100 system and column gel permeation. The main component, named PLP (Plantago asiatica L. polysaccharides) was obtained. Its purity and molecular weight (Mw) were determined by high performance gel permeation chromatography (HPGPC). Gas chromatography-mass spectrometry (GC-MS) was used to study the monosaccharide composition. The results showed that PLP is homogeneous. The Mw is about 891.3 kD. Arabinose, xylose, mannose and galactose are determined to be the components of PLP in the mol ratios of 5.6∶9.4∶1.3∶1.

Key words: polysaccharides from Plantago asiatica L, purification, molecular weight, monosaccharide composition

YIN Jun-Yi, NIE Shao-Ping, FU Zhi-Hong, XIE Ming-Yong. Isolation and Purification of Polysaccharide from Seeds of Plantago asiatica L. and Analysis of Its Monosaccharide Compositions[J]. FOOD SCIENCE, 2008, 29(9): 529-532.

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Isolation and Purification of Polysaccharide from Seeds of Plantago asiatica L. and Analysis of Its Monosaccharide Compositions

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