Abstract: β-D-glucans were extracted from the cell wall of spent brewer’s yeast (Saccharomyces cerevisiae) by alkali, acid -alkali or enzyme-alkali method, respectively. The basic components of the three different extracted products were analyzed. Paper chromatography and IR spectrum analysis showed that D-mannose was not found in all of them. The adsorptive properties of β-D-glucans on Zearalenone(ZEA) were determined by HPLC. It indicated that the absorptive capacity was different to β- D-glucans with different extracting methods. β-D-glucans obtained by enzyme-alkali method showed a relatively high adsorp- tion capacity for ZEA with a maximum adsorption of up to 2.296μg ZEA/mg glucan.

Key words: zearalenone； mycotoxin； brewer yeast； &beta, -D-glucan； adsorption；