Study on Technology of Extraction and Purification Superoxide Dismutase from Yellow Mealworm

FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (9): 281-283.

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Study on Technology of Extraction and Purification Superoxide Dismutase from Yellow Mealworm

WU Lei, CHEN Qing-Sen, LIU Jin-Sheng

1.Tianjin Key Laboratory of Food and Biotechnology,Department of Bioengineering,Tianjin University of Commerce,Tianjin 300134,China;2.School of Chemical-Engineering,Tianjin University,Tianjin 300072,China

Online:2007-09-15 Published:2011-11-22

Abstract

Abstract: In this paper,a kind of superoxide dismutase(SOD)was obtained from yellow worm powder.Two-step(NH4)2SO4 precipitation were used as the main extraction method and DEAE-Sepharose FF ion-exchange and Sephadex G200 volume exclude chromatography were used as purification methods.The purification factor was 78.3.The SDS-PAGE showed that the SOD was a homogenous protein.This enzyme was proved as Cu.Zn-SOD.This study provides a suitable technology for obtaining SOD from the yellow mealworm.

Key words: yellow mealworm, SOD, extraction, purification, chromatography

WU Lei, CHEN Qing-Sen, LIU Jin-Sheng. Study on Technology of Extraction and Purification Superoxide Dismutase from Yellow Mealworm[J]. FOOD SCIENCE, 2007, 28(9): 281-283 https://doi.org/

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Study on Technology of Extraction and Purification Superoxide Dismutase from Yellow Mealworm

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