Abstract: The optimal conditions for the extraction of blackberry red pigment from blackberry fruits were studied by orthogonal test, and also the isolation and purification of blackberry red pigment with adsorbent resins and the stability of blackberry red pigment were studied in this paper. Among the tested factors, temperature showed the most remarkable effect on pigment extracting, while the ratio of solid to solution, extraction times and duration showed minor effects. The optimal operation conditions were: the ratio of solid to solution 1 to 6, temperature 40～60℃, and extracting twice, with 15min each. Macroporous resins could be used to purify blackberry red pigment effectively. Resin AB-8 was excellent for the separation and purification of blackberry red pigment and 80% ethanol solution was the better elute solution. The pigment was sensitive to pH, fit for being used at acid condition(pH<4.0). It was stable when the processing temperature was under 80℃. So blackberry red pigment could be used in acid food processing.

Key words: blackberry red pigment, extraction, adsorbent resin, stability